English. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an antibiotic cassette flanked by a region homologous to the target locus into a strain that … A lateral part or side: the flank of a mountain. At the end of the process the integrated provirus is flanked by a direct repeat of cell sequences, similar to the repeats that flank DNA transposons. WikiMatrix. Flanking Region: For microsatellites, the flanking regions are the stretches of DNA outside the simple sequence tandem repeat (STR). How to use flank in a sentence. English English - Japanese English - Korean English - Spanish Japanese - English Spanish - English. The section of flesh on the body of a person or an animal between the last rib and the hip; the side. region [re´jun] a plane with more or less definite boundaries; called also regio. These shields are flanked by two male figures who function as bearers of a coat of arms. tr.v. These ITRs base pair to allow for synthesis of the complementary DNA strand. flank. In military tactics, a flanking maneuver, or flanking manoeuvre is a movement of an armed force around an enemy force's side/flank to achieve an advantageous position over it. flanking sequence. Foreign words are automatically translated to your language of choice. The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. Once the loxP-flanked sequence was excised by Cre-mediated recombination in a cell, Egfp was stably expressed under the control of ubiquitous hsc71-regulatory regions . It mediates higher transcription efficiency than the generally u,biological,biology supply,biology supplies,biology product It is the region of DNA that is not transcribed into RNA. Molecular Biology Tools ; Peptide Tools; Protein Tools ; Learning Center ; Research Applications; Reference Databases; KOL Seminar Series; 2020 Webinar Series; Express Cloning. jw2019. ‘Seated in the dock, flanked by prison officers, he adopted his customary stance - shoulders slouched forward, head bowed.’ ‘Two black leather sofas flanked a low table in front of the receptionist, and Wendy chose one.’ ‘Every computer desk I've had until recently was flanked … Rep and Cap are translated to produce multiple distinct proteins (Rep78, Rep68, Rep52, Rep40 - required for the AAV life cycle; VP1, VP2, VP3 - capsid proteins). Flank definition: An animal's flank is its side, between the ribs and the hip. “DNA cloning is a molecular biology technique which is used for the creation of exact copies or clones of a particular gene or DNA.” DNA cloning is the process of making multiple copies of a particular segment of DNA. Spliceosome-associated proteins and splicing mediators are colored blue and green, respectively. loxP flanked, Pro and Euk. Here are some examples: "Exons flank introns" "Generation of Direct Repeats in Host DNA Flanking a Transposon" etc.. n. 1. Another word for flanked. Define flank. Thus the natural DNA-repair mechanisms of the cell can be used to insert the desired genetic material, editing the genome of a target cell with high-precision. Thanks! loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH,The The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly modified version of the Em7 promoter. A DNA sequence located … The loxP-PGK-gb2-neo-loxP template encodes the neomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of … flank (flăngk) n. 1. adj., adj re´gional. The answer should be written using the space allocated below each question. flanked by. 4. a. b. What does it mean when DNA is 'flanking'? Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH,The loxP-PGK-gb2-neo-loxP template is designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells. This is the meaning of flox: flox (English)Origin & history I Abbreviation of "flanked by loxP" Verb flox (third-person singular simple present floxes, present participle floxing, simple past and past participle floxed) . 2) View the complete definition of any word or phrase using the toolbar dictionary. 2. The right or left side of a military formation: an attack on both flanks. Flank definition, the side of an animal or a person between the ribs and hip. The 5′ flanking region is a region of DNA that is adjacent to the 5′ end of the gene. 2. It occurs commonly among lower plants and invertebrate animals (particularly rotifers, aphids, ants, wasps, and bees) and rarely among higher vertebrates. 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